Dual‐modality in vivo monitoring of subventricular zone stem cell migration and metabolism
Identifieur interne : 002588 ( Main/Exploration ); précédent : 002587; suivant : 002589Dual‐modality in vivo monitoring of subventricular zone stem cell migration and metabolism
Auteurs : Francesca Cicchetti [Canada, États-Unis] ; Robert E. Gross [États-Unis] ; Jeff W. M. Bulte [États-Unis] ; Mary Owen [États-Unis] ; Iris Chen [États-Unis] ; Martine Saint-Pierre [Canada] ; Xukui Wang [États-Unis] ; Meixiang Yu [États-Unis] ; Anna-Liisa Brownell [États-Unis]Source :
- Contrast Media & Molecular Imaging [ 1555-4309 ] ; 2007-05.
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Abstract
Rat subventricular zone (SVZ) stem cells were labeled with superparamagnetic iron oxide particles (SPIO) to follow their fate and migratory potential with magnetic resonance imaging (MRI) and positron emission tomography (PET). Labeled cells were transplanted into either the right rostral migratory stream (RMS) or striatum of normal adult Sprague–Dawley rats and serially followed for 3 months. Minimal migration of the cells implanted into the striatum was observed after 3 weeks whereas SVZ cells implanted into the RMS migrated toward the olfactory bulb at 1 week post‐transplantation. PET studies of glucose metabolism using 18F‐FDG demonstrated enhanced glucose utilization in the striatum of transplanted animals. PET studies conducted 3 months after transplantation showed elevated accumulation of 11C‐raclopride (dopamine receptor type 2) and 11C‐CFT (dopamine transporter) binding in the striatal grafts. Implanted SVZ cells did not induce significant inflammation as identified by PET using 11C‐PK11195, a ligand detecting activated microglia. Histological analysis identified viable SPIO‐labeled cells (some of which were nestin‐positive) 7 weeks post‐transplantation, suggesting a prolonged presence of undifferentiated neural stem cells within transplants. In addition, double immunostaining for neuronal and astrocytic markers (NeuN and GFAP) indicated that differentiation into neuronal and astrocytic phenotypes also occurred. Thus, combining MRI and PET enables monitoring of cell migration and metabolism non‐invasively in vivo for extended periods of time. Copyright © 2007 John Wiley & Sons, Ltd.
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DOI: 10.1002/cmmi.138
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Morgan Department of Radiology and Radiological Science, Department of Chemical & Biomolecular Engineering, and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205</wicri:regionArea><wicri:noRegion>21205</wicri:noRegion></affiliation></author><author><name sortKey="Owen, Mary" sort="Owen, Mary" uniqKey="Owen M" first="Mary" last="Owen">Mary Owen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Simmons College, Boston, MA 02115</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Chen, Iris" sort="Chen, Iris" uniqKey="Chen I" first="Iris" last="Chen">Iris Chen</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiology, Massachusetts General Hospital, Boston, MA, 02114</wicri:regionArea><wicri:noRegion>02114</wicri:noRegion></affiliation></author><author><name sortKey="Saint Ierre, Martine" sort="Saint Ierre, Martine" uniqKey="Saint Ierre M" first="Martine" last="Saint-Pierre">Martine Saint-Pierre</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Centre de Recherche en Neurosciences, CHUL, RC‐9800, 2705 Boulevard Laurier, Québec</wicri:regionArea><wicri:noRegion>Québec</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xukui" sort="Wang, Xukui" uniqKey="Wang X" first="Xukui" last="Wang">Xukui Wang</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiology, Massachusetts General Hospital, Boston, MA, 02114</wicri:regionArea><wicri:noRegion>02114</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Meixiang" sort="Yu, Meixiang" uniqKey="Yu M" first="Meixiang" last="Yu">Meixiang Yu</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiology, Massachusetts General Hospital, Boston, MA, 02114</wicri:regionArea><wicri:noRegion>02114</wicri:noRegion></affiliation></author><author><name sortKey="Brownell, Anna Iisa" sort="Brownell, Anna Iisa" uniqKey="Brownell A" first="Anna-Liisa" last="Brownell">Anna-Liisa Brownell</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiology, Massachusetts General Hospital, Boston, MA, 02114</wicri:regionArea><wicri:noRegion>02114</wicri:noRegion></affiliation><affiliation wicri:level="2"><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>Francesca Cicchetti, Centre de Recherche en Neurosciences, CHUL, RC‐9800, 2705, Boulevard Laurier, Québec, Canada G1V 4G2.Anna‐Liisa Brownell, Department of Radiology, Bartlett Hall 504R, Massachusetts General Hospital, Boston, MA 02114</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Contrast Media & Molecular Imaging</title><title level="j" type="abbrev">Contrast Media Mol Imaging</title><idno type="ISSN">1555-4309</idno><idno type="eISSN">1555-4317</idno><imprint><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2007-05">2007-05</date><biblScope unit="volume">2</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="130">130</biblScope><biblScope unit="page" to="138">138</biblScope></imprint><idno type="ISSN">1555-4309</idno></series><idno type="istex">4F8AAAEC9F824B1D2B29BAD198E7FA993948FBB9</idno><idno type="DOI">10.1002/cmmi.138</idno><idno type="ArticleID">CMMI138</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1555-4309</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>dopamine</term><term>inflammation</term><term>magnetic resonance imaging (MRI)</term><term>positron emission tomography (PET)</term><term>superparamagnetic iron oxide particles</term><term>transplantation</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rat subventricular zone (SVZ) stem cells were labeled with superparamagnetic iron oxide particles (SPIO) to follow their fate and migratory potential with magnetic resonance imaging (MRI) and positron emission tomography (PET). Labeled cells were transplanted into either the right rostral migratory stream (RMS) or striatum of normal adult Sprague–Dawley rats and serially followed for 3 months. Minimal migration of the cells implanted into the striatum was observed after 3 weeks whereas SVZ cells implanted into the RMS migrated toward the olfactory bulb at 1 week post‐transplantation. PET studies of glucose metabolism using 18F‐FDG demonstrated enhanced glucose utilization in the striatum of transplanted animals. PET studies conducted 3 months after transplantation showed elevated accumulation of 11C‐raclopride (dopamine receptor type 2) and 11C‐CFT (dopamine transporter) binding in the striatal grafts. Implanted SVZ cells did not induce significant inflammation as identified by PET using 11C‐PK11195, a ligand detecting activated microglia. Histological analysis identified viable SPIO‐labeled cells (some of which were nestin‐positive) 7 weeks post‐transplantation, suggesting a prolonged presence of undifferentiated neural stem cells within transplants. In addition, double immunostaining for neuronal and astrocytic markers (NeuN and GFAP) indicated that differentiation into neuronal and astrocytic phenotypes also occurred. Thus, combining MRI and PET enables monitoring of cell migration and metabolism non‐invasively in vivo for extended periods of time. Copyright © 2007 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><country name="Canada"><noRegion><name sortKey="Cicchetti, Francesca" sort="Cicchetti, Francesca" uniqKey="Cicchetti F" first="Francesca" last="Cicchetti">Francesca Cicchetti</name></noRegion><name sortKey="Cicchetti, Francesca" sort="Cicchetti, Francesca" uniqKey="Cicchetti F" first="Francesca" last="Cicchetti">Francesca Cicchetti</name><name sortKey="Saint Ierre, Martine" sort="Saint Ierre, Martine" uniqKey="Saint Ierre M" first="Martine" last="Saint-Pierre">Martine Saint-Pierre</name></country><country name="États-Unis"><region name="Massachusetts"><name sortKey="Cicchetti, Francesca" sort="Cicchetti, Francesca" uniqKey="Cicchetti F" first="Francesca" last="Cicchetti">Francesca Cicchetti</name></region><name sortKey="Brownell, Anna Iisa" sort="Brownell, Anna Iisa" uniqKey="Brownell A" first="Anna-Liisa" last="Brownell">Anna-Liisa Brownell</name><name sortKey="Brownell, Anna Iisa" sort="Brownell, Anna Iisa" uniqKey="Brownell A" first="Anna-Liisa" last="Brownell">Anna-Liisa Brownell</name><name sortKey="Bulte, Jeff W M" sort="Bulte, Jeff W M" uniqKey="Bulte J" first="Jeff W. M." last="Bulte">Jeff W. M. Bulte</name><name sortKey="Chen, Iris" sort="Chen, Iris" uniqKey="Chen I" first="Iris" last="Chen">Iris Chen</name><name sortKey="Gross, Robert E" sort="Gross, Robert E" uniqKey="Gross R" first="Robert E." last="Gross">Robert E. Gross</name><name sortKey="Gross, Robert E" sort="Gross, Robert E" uniqKey="Gross R" first="Robert E." last="Gross">Robert E. Gross</name><name sortKey="Owen, Mary" sort="Owen, Mary" uniqKey="Owen M" first="Mary" last="Owen">Mary Owen</name><name sortKey="Wang, Xukui" sort="Wang, Xukui" uniqKey="Wang X" first="Xukui" last="Wang">Xukui Wang</name><name sortKey="Yu, Meixiang" sort="Yu, Meixiang" uniqKey="Yu M" first="Meixiang" last="Yu">Meixiang Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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